Renin, Prorenin and the Putative (Pro)renin Receptor
نویسندگان
چکیده
منابع مشابه
Renin, prorenin and the putative (pro)renin receptor.
Renin is an aspartic protease that consists of 2 homologous lobes. The cleft in between contains the active site with 2 catalytic aspartic residues.1 Unlike other aspartic proteases such as pepsin or cathepsin D, renin is monospecific and only cleaves angiotensinogen, to generate angiotensin (Ang) I. Ang I is the precursor of the active end-product of the renin-angiotensin system (RAS), Ang II....
متن کاملProrenin/renin and insulin resistance.
Recently we showed that fructose feeding in rats induced nonproteolytic activation of prorenin and subsequent angiotensin II production in skeletal muscle. In addition, a pharmacological inhibitor of prorenin/(pro)renin receptor interaction attenuated the development of insulin resistance. However, the inhibitor did not ameliorate the glucose intolerance in transgenic rats overexpressing the hu...
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In this issue of the journal, we feature two articles—one by Tadashi Inagami and the other by Pierre Corvol and Joel Menard—relating the story of the purification of renin. These investigators received the 1985 CIBA Award for Hypertension Research for biochemical studies leading to the isolation, amino acid structural and three-dimensional configuration, and the physiochemical and immunochemica...
متن کاملBinding properties of rat prorenin and renin to the recombinant rat renin/prorenin receptor prepared by a baculovirus expression system.
The rat recombinant renin/prorenin receptor (AB188298 in DDJB), which conjugated with FLAG epitope in its N-terminus, was expressed in a baculovirus expression system. The recombinant receptor, prepared from the cytoplasmic fraction of the insect cells, was identified by Western blotting using anti-FLAG antibody. Prorenin as well as renin bound to the receptor with different binding affinities....
متن کاملTwo - step Prorenin - Renin Conversion
Prorenin is an inactive form of the aspartic protease renin. Like pepsinogen, it is activated at low pH. The kinetics of acid activation of prorenin were studied in human amniotic fluid and plasma and in preparations of purified prorenin isolated from amniotic fluid and plasma. Conversion of prorenin (pR) into active renin (R) appeared to be a two-step process involving the generation of an int...
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ژورنال
عنوان ژورنال: Hypertension
سال: 2005
ISSN: 0194-911X,1524-4563
DOI: 10.1161/01.hyp.0000186329.92187.2e